Tretiakova Polina Yakovlevna (PhD student, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy (Moscow))
Soloviev Aleksandr Aleksandrovich (Dr. Biol. Sc., The All-Russia Research Institute of Agricultural Biotechnology (Moscow))
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Host-induced gene silencing (HIGS) has been shown to be an effective tool when studying interactions between pathogens and crops. To study head blight on wheat caused by Fusarium culmorum, γ-vector of barley stripe mosaic virus (BSMV) was modified so it carried the fragment of the tri5 gene enabling dsRNA-mediated transient silencing. Capped viral RNAs were synthesized to perform an infection of young wheat plants. The infection efficiency by virus/viral construct amounted to 98%. Application of the γ-PDS vector resulted in colorless stripes and areas on plant leaves proving the effectiveness of the silencing machinery. The γ-TRI5 vector was developed to test the aggressiveness of F. culmorum while suppressing its ability to produce trichothecene mycotoxins that are a potential virulence factor on wheat.
Keywords:head blight, virus, wheat, BSMV, VIGS, HIGS, Fusarium culmorum, tri5, trichothecene mycotoxins, silencing, RNA interference, dsRNA, transient expression, pathosystem, infection.
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Citation link:
Tretiakova P. Y., Soloviev A. A. HIGS in wheat-Fusarium culmorum pathosystem: modification of barley stripe mosaic virus vector // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2020. -№06. -С. 32-42 DOI 10.37882/2223-2966.2020.06.37 |
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