Burlakova Lyubov A. (Postgraduate Student of the Department of Maxillofacial Surgery and Surgical Dentistry, Peoples' Friendship University of Russia, Moscow, Russia)
Gizinger Oksana A. (Grand PhD in Biological Sciences, Professor of V.S. Kiktenko Department of Microbiology, Peoples' Friendship University of Russia Moscow, Russia)
Muraev Alexander A. (Grand PhD in Medical Sciences, Professor of the Department of Maxillofacial Surgery and Surgical Dentistry Peoples' Friendship University of Russia, Moscow, Russia )
Delidova Ekaterina V. (PhD, dentist-surgeon, periodontist, implantologist, Denta Vita Prestige LLC, Moscow, Russia)
Ivanov Sergey Yu. (Corresponding Member of the Russian Academy of Sciences, Professor, Grand PhD in Medical Sciences, Head of the Department of Maxillofacial Surgery of the I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia )
Yamurkova Nina F. (Grand PhD in Medical Sciences, Associate Professor, Honored Doctor of the Russian Federation, maxillofacial surgeon of the highest category of City Clinical Hospital No. 39, Nizhny Novgorod, Russia)
Sergeev Yuri A. (PhD, Orthopedic Dentist, Assistant at the Department of Pharmacology, Stavropol State Medical University, Stavropol, Russia)
Dolgalev Alexander A. (Grand PhD in Medical Sciences, Professor, Department of General Dentistry and Pediatric Dentistry, Stavropol State Medical University, Stavropol, Russia )
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As part of our study, microbial communities in patients with peri-implantitis were evaluated using diagnostic methods such as PCR, 16s metagenomic sequencing and GC-MS.
The aim of the study was to evaluate the correlation of the results obtained as a result of PCR, 16s metagenomic sequencing and GC-MS methods.
Materials and methods. The saliva of 10 patients with chronic peri-implantitis aged 18 to 80 years was studied.
Results. The obtained PCR data confirmed the presence of major periodontal pathogens such as P. endodontalis, P. gingivalis, A. actinomycetemcomitans, T. denticola, P. intermedia, T. forsythia and F. nucleatum. Metagenomic sequencing revealed not only the presence but also the number of these bacteria, demonstrating their dominance in the microbiome. GC-MS analyses revealed elevated concentrations of certain bacterial genera and showed that 54.25% of microorganisms were conditionally pathogenic, which highlights the violation of the microecological status in the oral cavity of patients. The data obtained indicate a decrease in the diversity of genera and species in the microbiome, which supports the theory that the maintenance of the inflammatory process in peri-implantitis is associated with dysbiotic changes in the microbial community, leading to the dominance of pathogenic species and the progression of the disease.
Keywords:gas chromatography-mass spectrometry, oral microflora, periodontopathogenic microflora, peri-implantitis
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Citation link:
Burlakova L. A., Gizinger O. A., Muraev A. A., Delidova E. V., Ivanov S. Y., Yamurkova N. F., Sergeev Y. A., Dolgalev A. A. POSSIBILITIES OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY OF MICROBIAL MARKERS IN THE DIAGNOSIS OF PERIODONTAL MICROFLORA IN THE ORAL CAVITY // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2024. -№11/2. -С. 12-17 DOI 10.37882/2223-2966.2024.11-2.05 |
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