Shao Chengyue (Belarusian State University
Postgraduate student at the department of molecular biology, faculty of biology
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Yevtushenkov Anatoly Nikolaevich (Belarusian State University
Doctor of science (biology), full professor of the department of molecular biology, faculty of biology
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Potato samples with blackleg disease were collected in the Belarus region, and bacteria were extracted. Biochemical and molecular genetic analyses revealed that the characteristics of seven bacterial strains were consistent with those of Pectobacterium brasiliense. Bacterial genomes were extracted and amplified using Pectobacterium brasiliense-specific primers BR1f and L1r . One sample was selected for gene extraction and whole genome sequencing, and its genome showed similarity to Pectobacterium brasiliense. Pathogen identification was confirmed by phylogenetic analysis using 16S RNA genes. After plant inoculation, the same symptoms were observed on potato stems, potato tubers and carrot tubers, satisfying Koch's hypothesis. Pectobacterium brasiliense infection has been reported in Brazil, the USA, Russia, and China.
Keywords:Potato, blackleg disease, potato soft rot, Pectobacterium brasiliense.
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Citation link:
Shao C. , Yevtushenkov A. N. MOLECULAR GENETIC ANALYSIS OF PECTOBACTERIUM BRASILIENSE ISOLATED FROM PLANT SAMPLES IN BELARUS // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2024. -№11. -С. 19-25 DOI 10.37882/2223-2966.2024.11.44 |
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