Tochilina Anna Georgievna (Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology
PhD, senior researcher laboratory of the human microbiome and means of its correction; Privolzhsky Research Medical University
Associate Professor, Department of Epidemiology, Microbiology and Evidence-Based Medicine
)
Belova Irina Viktorovna (Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology
PhD, leading researcher laboratory of the human microbiome and means of its correction; Privolzhsky Research Medical University
Associate Professor, Department of Epidemiology, Microbiology and Evidence-Based Medicine
)
Molodtsova Svetlana Borisovna (Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology
Researcher laboratory of the human microbiome and means of its correction
)
Kropotov Vasily Sergeevich (Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology
PhD, senior researcher laboratory of the human microbiome and means of its correction; Privolzhsky Research Medical University
Associate Professor, Department of Epidemiology, Microbiology and Evidence-Based Medicine
)
Solovyova Irina Vladlenovna (Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology
Doctor of biological sciences, leading Researcher Head of laboratory of the human microbiome and means of its correction
)
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Introduction.
Microbiological monitoring belongs to the most important components of the epidemiologic safety system and allows to observe the variability of epidemically significant microorganisms, their features and circulation. In modern conditions the representatives of ESKAPE group are in the zone of special attention, which often cause hospital infections and complicate the course of the main disease.
Aims and objectives.
Characterization of phenotypic properties and molecular-genetic features of S. aureus strains, assimilated with CAIC, circulating in a multidisciplinary hospital
Materials and Methods.
Bacterial identification was performed by MALDI TOF mass spectrometry (Autoflex speed Bruker, Biotyper software); bacterial sensitivity to antibiotics was studied by the disc-diffusion method, and was evaluated according to the criteria of EUCAST v. 13.0. 13.0. Whole-genome sequencing was performed on the MiSeq platform “Illumina Inc.” (USA), processing was performed by the programs Spades v. 3.11.1 and Prokka v1.12. Full genome sequences were analyzed using PubMLST, VFDB, CARD web platforms, SCCmecFinder, VirulensFinder and PathogenFinder programs.
Results.
S. aureus strains circulating in a large hospital and associated with CAIC were found to belong to ten different sequencing types - S. aureus ST8, ST45, ST5, ST1, ST398, ST97, ST6, ST707, ST12, ST1027 with predominance of S. aureus ST8 t008 SCCmec IV. .
Conclusion. As a result of this work, the strains of S. aureus associated with CAIC circulating in a multidisciplinary hospital have been characterized in detail.
Keywords:S. aureus, CAIC, full genome sequencing, microbiologic monitoring.
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Citation link:
Tochilina A. G., Belova I. V., Molodtsova S. B., Kropotov V. S., Solovyova I. V. ANALYSIS OF THE GENETIC CHARACTERISTICS OF S. AUREUS STRAINS CIRCULATING IN A MULTIDISCIPLINARY HOSPITAL // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2024. -№12. -С. 19-23 DOI 10.37882/2223-2966.2024.12.35 |
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