Kapralov Sergey Vladimirovich (Doctor of Medicine Sciences, associate professor, Head of the Department of Faculty Surgery and Oncology, Saratov State Medical University named after V.I. Razumovsky)
Polidanov Maxim Andreevich (research department specialist, assistant of the Department of Biomedical Disciplines, University «Reaviz», Saint Petersburg, Russian Federation)
Volkov Kirill Andreevich (Saratov State Medical University named after V.I. Razumovsky, Saratov, Russian Federation)
Vysotskii Lev Ilyich (Saratov State Medical University named after V.I. Razumovsky, Saratov, Russian Federation)
Abramov Anton Maksimovich (Saratov State Medical University named after V.I. Razumovsky, Saratov, Russian Federation)
Marchenko Vladislav Sergeevich (resident physician of the Department of Urology, Saratov State Medical University named after V.I. Razumovsky, Saratov, Russian Federation)
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Objective. Experimentally justify the method of treatment of portal hypertension syndrome in liver cirrhosis. Materials and methods. A series of experiments were performed on white male rats of the Standard breed in the number of 80 animals, weighing 200±50 g. Beforehand, the laboratory animals were modeled liver cirrhosis according to the previously developed technique, the essence of which was intraoperative injection of 0.3-0.5 ml of 60-79% ethanol solution under the glisson capsule into the liver parenchyma of the animal. Due to this technique cirrhosis was modeled within 14 days from the experiment in 100% of animals and had expressed morphological (macro- and microscopic) signs. Further laser non-directed fenestration of the cirrhosis zone under the influence of a solid-state laser (laser device Lasermed 1-10 (Russia), near-infrared range) with the wavelength of 1064 nm, power 7 - 8 W was applied to the changed area of the liver by creating non-directed laser channels on the changed fragment of the liver. After 14 days the animals were taken out of the experiment, the fragments of the altered liver after laser exposure were dissected. For histological examination the liver pieces were preliminary fixed in 12% formalin, paraffinized, then cut on a microtome and stained with hematoxylin and eosin, as well as Van-Gizon and subsequently examined under the microscope «Olympus» «BX43F» (Japan). Results. When laser fenestration was applied to the cirrhotic liver during 14 days the manifestations of metabolism-synthetic and proliferative activity in the liver parenchymatous cells significantly increased, involution of sclerotic changes in the liver by the 21st day after laser revascularization was traced, and, accordingly, the process of neoangiogenesis significantly increased. Conclusions. The study of microchannels morphology at different terms showed that at early terms - a day or two - the channel formation under ultrasound control with coagulation necrosis and absence of hemorrhage into parenchyma occurs (the main advantage - safety of the method); at the term of 14 days - neoangiogenesis and appearance of young hepatocytes in microchannels outside hepatic lobules reliably occurs; and at the term of 21 days and more - liver regeneration with lobules restoration.
Keywords:Liver cirrhosis, experimental modeling, portal hypertension syndrome in cirrhosis, laser fenestration, liver regeneration
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Citation link:
Kapralov S. V., Polidanov M. A., Volkov K. A., Vysotskii L. I., Abramov A. M., Marchenko V. S. EXPERIMENTAL SUBSTANTIATION OF THE METHOD OF TREATMENT OF PORTAL HYPERTENSION SYNDROME IN LIVER CIRRHOSIS // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2025. -№01. -С. 201-206 DOI 10.37882/2223-2966.2025.01.20 |
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