Gavrukov Dmitry Sergeyevich (Surgeon, Head of Surgical Department № 4, Yudin City Clinical Hospital, Moscow, Russian Federation)
Kapralov Sergey Vladimirovich (Doctor of Medicine Sciences, associate professor, Head of the Department of Faculty Surgery and Oncology, Saratov State Medical University named after V.I. Razumovsky)
Polidanov Maxim Andreevich (research department specialist, assistant of the Department of Biomedical Disciplines, University «Reaviz», Saint Petersburg, Russian Federation)
Volkov Kirill Andreevich (Saratov State Medical University named after V.I. Razumovsky, Saratov, Russian Federation)
Tashukhozhaeva Diana Takhirovna (Candidate of Medical Sciences, Associate Professorof the Department of Obstetrics and Gynecology, Medical University «Reaviz», Saratov, Russian Federation)
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The aim of the study is to experimentally justify the method of evaluation of the technique of abdominal wall alloplasty operation. Materials and methods. 40 laboratory animals - rats of ‘Standard’ breed, weighing 450±50 g were used as biological models in the experiment. Alloplasty with fixed and unfixed antemuscular and retromuscular location of the mesh graft (Esfil, RF) was performed under general anaesthesia in aseptic conditions in the experimental operating room. We formed 4 observation groups (10 experimental rats in each group) depending on the method of alloplasty. In the 1-st group alloplasty was performed retromuscularly with fixation of the retinal graft, in the 2-nd group alloplasty was performed retromuscularly without fixation of the retinal graft; in the 3-rd group alloplasty was performed antemuscularly with fixation of the retinal graft, in the 4-th group alloplasty was performed antemuscularly without fixation of the retinal graft. On the 14th day the material was taken for biomechanical and morphological studies. The biomechanical properties were assessed by means of the tearing machine «INSTRON-5944». Tissue samples from the abdominal wall fragments containing grafts were subjected to the morphological study. The slices were stained by the method of ORB («orange-red-blue») modified by D.D. Zerbino and L.L. Lukasevich (1993). The preparations were examined and photographed under a light microscope «Leica Scope A1» with an integrated digital camera «Leica DC-500» at a magnification of 100 times. Results of the study. On the basis of the conducted experimental study, it can be concluded that morphological signs of collagen immaturity, cellular pattern of leukocytic infiltration of the postoperative scar are unfavourable signs worsening the quality of ventroplasty. On the contrary, morphological signs of mature collagen of the postoperative scar, minimal leukocytic infiltration, presence of fibroblasts in the scar testify to the favourable character of the wound process and sufficient quality of the performed ventroplasty. Conclusions. Thus, the data on the importance of morphological examination of the postoperative scar on the 14th day after the operation are confirmed by the subsequent testing of the operated abdominal wall on the tearing machine: at unfavourable morphological picture there is a decrease in the strength characteristics of the abdominal wall.
Keywords:Ventral hernias; prosthetic alloplasty; biomechanical properties of mesh grafts; tearing machine; morphological evaluation
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Citation link:
Gavrukov D. S., Kapralov S. V., Polidanov M. A., Volkov K. A., Tashukhozhaeva D. T. EXPERIMENTAL SUBSTANTIATION OF THE METHOD OF EVALUATION OF THE TECHNIQUE OF ABDOMINAL WALL ALLOPLASTY OPERATION PERFORMANCE // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2025. -№04. -С. 188-192 DOI 10.37882/2223-2966.2025.04.04 |
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