Zimina Anastasia Alekseevna (Intern researcher of the Department of Biology and General Genetics
First Moscow State Medical University I.M. Sechenov
(Sechenov University)
)
Grinev Alexander Borisovich (PhD, associate professor of the Department of Biology and General Genetics
First Moscow State Medical University I.M. Sechenov
(Sechenov University)
)
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In this work, a comprehensive analysis of sequencing artifacts generated using Oxford Nanopore Technology (ONT) is presented, exemplified by the erroneous detection of the free-living amoeba Naegleria fowleri in blood samples from patients with malaria (Plasmodium falciparum). In all four examined samples, sequences demonstrating nearly 100% identity with the N. fowleri genome according to BLAST analysis were identified. However, subsequent multi-level verification, including phylogenetic analysis, open reading frame (ORF) prediction, and three-dimensional protein structure modeling using AlphaFold3, made it possible to establish the artifactual nature of these findings. The results obtained underscore the critical importance of comprehensive validation of ONT data in clinical studies, where false-positive results can lead to serious diagnostic errors. Particular attention is paid to the analysis of potential sources of artifacts, including systematic sequencing errors, characteristics of bioinformatic data processing, and issues with reference databases.
Keywords:Oxford Nanopore Technology, sequencing artifacts, Naegleria fowleri, bioinformatic analysis, false-positive results, primary amoebic meningoencephalitis, AlphaFold3, BLAST analysis
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Citation link:
Zimina A. A., Grinev A. B. OXFORD NANOPORE TECHNOLOGY DATA SEQUENCING ARTIFACTS: FALSE-POSITIVE DETECTION OF NAEGLERIA FOWLERI AND ITS BIOINFORMATICS VALIDATION // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2025. -№06/2. -С. 29-32 DOI 10.37882/2223-2966.2025.06-2.12 |
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