Kuvika Igor Sergeevich (Postgraduate student, Institute of Animal husbandry named after academy member L.K. Ernst)
Zinovieva Natalia Anatolyevna (Doctor of Biology, Professor,
Director of the Federal State Budgetary Budgetary Institute of Animal husbandry named after academy member L.K. Ernst
)
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The black soldier fly (Hermetia illucens) is currently one of the most promising genetic resources, providing not only a lot of alternative protein and fat, which is necessary for animal feed, but also able to effectively bioconvert organic waste generated during the production of crop and livestock products. In 2023, it was included in the list of agricultural products by the Government of the Russian Federation. But at the moment, it is very difficult to find information on DNA extraction methods from the black soldier fly in the domestic literature. The purpose of this study is to compare the effectiveness of known methods for isolating DNA from the black soldier fly and to assess the effectiveness based on the results obtained. It has been established that the most effective method is the perchlorate method, since when using it, it is possible to isolate the highest molecular weight DNA than when using the commercial DNA-Extran-2 kit. Evaluation criteria include DNA yield, purity, integrity, and suitability for subsequent applications such as PCR and sequencing. The results show that when using the perchlorate extraction method, the DNA is more intact than when using the commercial DNA-Extrav-2 kit, which is more suitable for further research and work with this animal. This study provides insights into the optimal extraction methods for various molecular applications, contributing to future research.
Keywords:black soldier fly, Hermetia illucens, DNA extraction, sodium perchlorate, DNA-Extran-2.
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Read the full article …
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Citation link:
Kuvika I. S., Zinovieva N. A. COMPARISON OF THE EFFICIENCY OF DNA ISOLATION METHODS FROM THE BLACK LION FLY HERMETIA ILLUCENS // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2025. -№09. -С. 34-37 DOI 10.37882/2223-2966.2025.09.17 |
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