Polidanov Maxim Andreevich (research department specialist, assistant of the Department of Biomedical Disciplines, University «Reaviz», Saint Petersburg, Russian Federation)
Kapralov Sergey Vladimirovich (Doctor of Medicine Sciences, associate professor, Head of the Department of Faculty Surgery and Oncology, Saratov State Medical University named after V.I. Razumovsky)
Masliakov Vladimir Vladimirovich (Doctor of Medical Sciences, Professor of the Department of Mobilization Preparation of Public Health and Disaster Medicine, Saratov State Medical University named after V.I. Razumovsky, Saratov, Russia; Doctor of Medical Sciences, Professor, Professor of the Department of Surgical Diseases, Medical University «Reaviz», Saratov, Russia)
Volkov Kirill Andreevich (Saratov State Medical University named after V.I. Razumovsky, Saratov, Russian Federation)
Petrunkin Rodion Pavlovich (Private University «Reaviz», Saint Petersburg, Russia; )
Kashikhin Andrei Andreevich (Private University «Reaviz», Saint Petersburg, Russia; )
| |
The aim of the study is to develop a reliable and reproducible method of modeling antibiotic-associated colitis in laboratory animals to study the mechanisms of development of this disease, evaluate the effectiveness of potential therapeutic interventions, and search for new approaches to treatment and prevention. Materials and Methods. The study was carried out on 24 female laboratory rats of “Standard” breed weighing 200±50 g. The animals were given general anesthesia 30 minutes before the experiment. For modeling of antibiotic-associated colitis a solution of a broad-spectrum antibiotic (Amoxiclav, dose 2 ml/g, due to a large number of complications of this group of antibiotics on the gastrointestinal tract system) was used, which was injected into the rectum through the anus. Results. It was found that the development of antibiotic-associated colitis obtained using the developed method in all experimental animals was identical in the nature of damage to the mucous membrane of the rectum. The claimed method is simple enough, reproducible in carrying out, does not require carrying out in the conditions of specially equipped premises. Conclusions. Thus, the obtained experimental data confirm that the developed method allows by introducing a solution of a broad-spectrum antibiotic (Amoxiclav 2 ml/g) into the wall of the rectum and its lumen through the anus to provoke the development of antibiotic-associated colitis, which is very relevant at present and determines the prospects of its wide use for research purposes.
Keywords:Antibiotic-associated colitis, Clostridium difficile culture, experimental modelling, female rats
|
|
| |
|
Read the full article …
|
Citation link:
Polidanov M. A., Kapralov S. V., Masliakov V. V., Volkov K. A., Petrunkin R. P., Kashikhin A. A. EXPERIMENTAL SUBSTANTIATION OF THE METHOD OF MODELING ANTIBIOTIC-ASSOCIATED COLITIS IN LABORATORY ANIMALS // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2025. -№03. -С. 218-222 DOI 10.37882/2223-2966.2025.03.37 |
|
|
